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. 2004 Jul;186(13):4399–4401. doi: 10.1128/JB.186.13.4399-4401.2004

FIG. 2.

FIG. 2.

Components of the dual regulation system. The host strain is WH207λtet50. tetR(BD)-i1.9 [encoding the 4-DDMA-ATC-specific mutant TetR(BD) H64K L131I S135L] is constitutively expressed from plasmid pWH628 and binds tetO located upstream of lacZ on the chromosome. XynB, expressed from pWH628, is regulated via tetO-4C that is bound by TetR(B)-4C. TetR(B)-4C is constitutively expressed from pWH1925-tetR-4C. ori codes for origin of replication, cat codes for chloramphenicol acetyltransferase, bla codes for β-lactamase, xynB codes for β-Xyl, and lacZ codes for β-Gal.