TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| E. coli DH5α | recA1 endA1 gyrA96 thi relA1 hsdR17(rK− mK+) supE44 φ80dlacZΔM15 ΔlacU169 | 3 |
| B. subtilis 168 | trpC2 | Laboratory stock |
| E. coli WH207 | galK rpsL thi ΔlacX74 recA13 | 17 |
| E. coli WH207λtet50 | Tn10 tetA-lacZ transcriptional fusion, bet+gam+cIII+cI+lacZ+ | 15 |
| Plasmids | ||
| pWH1925-tetR-4C | Apr, pMB1 tetR-4C | This study |
| pWH1925-Δ | pWH1925-tetR-4C without tetR gene | 12a |
| pWH1411-i1.9 | CmrtetR-H64K L131I S135L | 12 |
| pWH628 | Cmr, pWH1411-i1.9 with tetO-4C-xynB region | This study |
| pWH627 | pWH628 without tetR gene | This study |