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. 2014 Oct 31;9(10):e110737. doi: 10.1371/journal.pone.0110737

Figure 3. Normal lactation in mammary glands from dab2 mosaic knockout mice.

Figure 3

(A) Mammary glands from 4-month old, 5-day lactating mice of dab2 (f/df):Meox2-Cre genotype show mosaic Dab2 immunostaining. (B) Dab2 was absent in more than 99% of mammary epithelial cells from mice of dab2 (f/df):Sox2-Cre genotype. (C) DNA extracted from tail tissues was used to genotype flox (f) and delta flox (df) alleles of the dab2 gene in dab2 (+/df): Sox2-Cre, dab2 (f/df):Meox2-Cre, and dab2 (f/df):Sox2-Cre. (D) Milk was harvested from dab2 heterozygous (dab2 (+/df):Sox2-Cre) and knockout (dab2 (f/df):Sox2-Cre) mice. Total proteins were resolved by SDS-PAGE and stained with Coomassie blue. (E) Specific proteins, beta-casein and GC-globin (vitamin D binding protein), were analyzed by immunoblot.