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Allelic exchange in mc22670, a fas1 merodiploid strain of M. smegmatis, results in mc22700, an M. smegmatis strain bearing M. tuberculosis fas1. (A) Map of the fas1 genomic region of wild-type and mutant strains of M. smegmatis (following allelic exchange) and wild-type M. tuberculosis fas1, showing AflIII sites. (B) Southern blot of AflIII-digested genomic DNA from different strains probed with the fragments shown in panel A. Mtb, M. tuberculosis; Msmg, M. smegmatis.
