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. 2014 Oct 31;9(10):e111266. doi: 10.1371/journal.pone.0111266

Figure 1. BAFF expression and secretion are up-regulated in Poly (I:C)- and IFN-γ-stimulated rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) and systemic sclerosis (SSc) human dermal fibroblast (HDF).

Figure 1

A, C. BAFF mRNA expression was determined by RT-qPCR in NFLS(n = 4) and RAFLS(n = 4) (A) or NHDF(n = 3) and SScHDF (n = 4) (C) stimulated (depending of the cell types) with BLP (1 µg/ml), LPS (1 µg/ml), Poly (I:C) (10 µg/mL) or IFN-γ (0.1, 1 or 5 ng/mL) for 72 h. Results were normalized to Gapdh and expressed as fold change compared with samples from cells incubated in medium. B, D. BAFF release was quantified by ELISA in culture supernatants of NFLS (n = 4) and RAFLS(n = 4) (B) or NHDF(n = 3) and SScHDF (n = 4) (D) in the same conditions as panels A and C. Data are expressed as the mean of triplicate samples ± SEM. *p<0.05, **p<0.01, ***p<0.001.