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. 2014 Oct 31;9(10):e111266. doi: 10.1371/journal.pone.0111266

Figure 3. miR-30a-3p directly targets the 3′UTR of BAFF mRNA.

Figure 3

A. Luciferase reporter constructs with wild-type or mutated (for miR-30-3p binding sites) BAFF 3′UTR were generated. B. HEK293 cells were transiently co-transfected with reporter constructs and with miR-30a-3p mimic (20 pM). Firefly Luciferase activities were measured 48 h after transfection and normalized to Renilla Luciferase expressed by the control psi-CHECK-2 vector devoid of 3′UTR sequences. Data are expressed as the mean of triplicate samples ± SEM and are representative of three independent experiments. **p<0.01.