FIG. 3.

Conditioned medium from A164, but not A164Δ10, rescues swarm motility of A164Δ10. A164Δ10 was inoculated onto swarm plates prepared with conditioned medium and incubated for 24 h at 37°C. Conditioned medium incubated with isopropanol alone had no effect on swarming (data not shown). (A) Conditioned medium obtained from A164 (left) or A164Δ10 (right). (B) Conditioned medium obtained from A164 and preincubated with PMSF at a final concentration of 140 (left) or 280 (right) μg/ml, respectively.