. 2004 Jul;186(13):4159–4167. doi: 10.1128/JB.186.13.4159-4167.2004

TABLE 2.

Plasmids and E. coli strains

Plasmid or strain	Description or genotype	Source(s) and/or reference
Plasmids
pCR2.1-TOPO	Cloning vector, Amp^r Kan^r	Invitrogen (29)
pNNB194	Shuttle vector, hybrid between pE194 and pBluescript II SK+, Erm^r Amp^r	Bacillus Genetic Stock Center (1E18), Stratagene (40)
pNNB194-Δepr	epr deletion vector, Erm^r Amp^r	This work
pNNB194-ΔsrfAC	srfAC deletion vector, Erm^r Amp^r	41
pNNB194-ΔwprA	wprA deletion vector, Erm^r Amp^r	This work
pNNB194-Δbpr	bpr deletion vector, Erm^r Amp^r	This work
pNNB194-Δvpr	vpr deletion vector, Erm^r Amp^r	This work
pNNB194-Δmpr	mpr deletion vector, Erm^r Amp^r	This work
pNNB194-ΔnprE	nprE deletion vector, Erm^r Amp^r	41
pNNB194-ΔaprE	aprE deletion vector, Erm^r Amp^r	41
pNNB194-ΔamyE	amyE deletion vector, Erm^r Amp^r	41
pNNB194-ΔsigF	sigF deletion vector, Erm^r Amp^r	41
pNNB194-Δsfp	sfp deletion vector, Erm^r Amp^r	This work
pNNB194-Δhag	hag deletion vector, Erm^r Amp^r	This work
pNNB194-srfACwt	srfAC replacement vector, Erm^r Amp^r	This work
E. coli strains
TOP10	F⁻mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(Str^r) endA1 nupG	Invitrogene (13)
JM101	supE thi-1 D(lac-proAB) [F′ traD36 proAB lacI^qZDM15]	Stratagene (48)