TABLE 3.
Primers used for splicing by overlapping extensiona
| Plasmid | Primer ID | Sequence (5′-3′) |
|---|---|---|
| pNNB194-Δwpr | CLJe7 | GGAATTCCAAAGCTGCAGCGGCCGGCGCG |
| CLJe8 | GAAGATCTCGTATACTTGGCTTCTGCAGCTGC | |
| CLJe9 | GAAGATCTGGTCAACAAGCTGGAAAGCACTC | |
| CLJe10 | CCCAAGCTTCGTGACGTACAGCACCGTTCCGGC | |
| pNNB194-Δbpr | bpr3Eco | GAATTCAAAGTCTTTTTCCCTATTGC |
| bpr4iSfiBamNot | GCGGCCGCGGATCCGGCCCTTAAGGCCTTTTGATATTCCGTCTTAGC | |
| bpr5SfiBamNot | GGCCTTAAGGGCCGGATCCGCGGCCGCCTTCTCCTGGCACAACGGTA | |
| bpr6iKpn | GGTACCGCGGCATACGAATGGTAAAC | |
| pNNB194-Δvpr | vpr3Eco | GAATTCCAATAAATGGTCCAAATGAC |
| vpr4iSfiBamNot | GCGGCCGCGGATCCGGCCCTTAAGGCCTGTGACGTTCGGGTACACTG | |
| vpr2SfiBamNot | GGCCTTAAGGGCCGGATCCGCGGCCGCCGAAGAACCTTTCACTGTT | |
| ywcH1Kpn | GGTACCTCCGGATGTGTGGCTGCTC | |
| pNNB194-Δmpr | mpr3Eco | GAATTCATCGATACGGGTGGTTGAC |
| mpr4iSfiBamNot | GCGGCCGCGGATCCGGCCCTTAAGGCCTATCGTCGAAGCCCATCCAT | |
| mpr5SfiBamNot | GGCCTTAAGGGCCGGATCCGCGGCCGCTTGGGAACAAGGGTGACGAA | |
| mpr6iKpn | GGTACCTCCTCCGGGTTTGATTGATA | |
| pNNB194-Δsfp | Sfp1A | GAATTCCGCGTGATTCACTGATGATGC |
| Sfp1B | AGTCTGTCTGCTCGTCCTTGTCTATGACTGAGCGAACGAGCAC | |
| Sfp1C | TGTGCTCGTTCGCTCAGTCATAGACAAGGACGAGCAGACAGAC | |
| Sfp1D | GGATCCCAAGCCATTCGAACTCACAGG | |
| pNNB194-Δhag | Hag1A | GATATCGCGTTATCCAGCGATGTGATC |
| Hag1B | GCACCAAGCTTAGCACGTTGAGAGCTTGAACAACTAGCTCACG | |
| Hag1C | TCGTGAGCTAGTTGTTCAAGCTCTCAACGTGCTAAGCTTGGTG | |
| Hag1D | GGATCCCAGGTTGTAACGTAGTGAGCC | |
| pNNB194-srfACwt | srfC1 | AAGCTTTGAATGGGTGTGG |
| srfC4i | CTGACATGAGGCACTGAC |
a
The primers used to construct the plasmids described by A. Sloma and L. Christianson (41) are listed therein.