TABLE 1.
Bacterial strains and plasmids used for this study
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| S. enterica | ||
| 14028s | Wild type | 9 |
| EG13927 | ΔpmrC1::Cmr, partial deletion (1,096 bp of the 1,641-bp pmrC gene) | This study |
| EG14590 | ΔpmrC1.1, Cmr removed from strain EG13927 | This study |
| EG13633 | ΔpmrC2::Cmr, complete deletion of the pmrC open reading frame | This study |
| EG9241 | pbgP1::MudJ | 40 |
| EG14372 | pbgP1::MudJ ΔpmrC1 | This study |
| EG14375 | pbgP1::MudJ ΔpmrC2 | This study |
| EG9492 | pmrA505 zjd::Tn10d-Cmr | 12 |
| EG9868 | pmrA505 pbgP1::MudJ zjd::Tn10d-Cmr | 12 |
| EG14367 | pmrA505 ΔpmrC::Kmrzjd::Tn10d-Cmr | This study |
| EG14368 | pmrA505 ΔpmrC1 zjd::Tn10d-Cmr | This study |
| EG14369 | pmrA505 pbgP1::MudJ ΔpmrC1 zjd::Tn10d-Cmr | |
| EG7139 | pmrA::Cmr | This study |
| EG14286 | phoN::Cmr | 41 |
| EG14656 | EG14590 containing pBAC108L plasmid | This study |
| EG14595 | EG14590 containing ppmrC plasmid | This study |
| EG14592 | EG14590 containing ppmrCFLAG plasmid | This study |
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17recA1 endA1 gyrA96 thi-1 relA1 | (18) |
| Plasmids | ||
| pBAC108L | Mini-F, Cmr | 38 |
| ppmrC | pBAC108L harboring pmrC coding region with its own promoter | This study |
| ppmrCFLAG | pBAC108L harboring the pmrC coding region with its own promoter and harboring sequences encoding a FLAG epitope sequence at the 3′ end | This study |
| pKD3 | repR6K γ FRT Cmr FRT Apr | 6 |
| pKD4 | repR6K γ FRT Kmr FRT Apr | 6 |
| pKD46 | reppSC101ts paraBAD γ β Exo Apr | 6 |
| pCP20 | reppSC101ts Cmr cI857 λPR Apr | 3 |
| pCE36 | repR6Kγ FRT lacZY this Kmr | 8 |
| pCL1920 | reppSC101ts Spr | 27 |
| pPmrC150 | pCL1920 harboring truncated pmrC150 | This study |
| pPmrC181 | pCL1920 harboring truncated pmrC181 | This study |
| pPmrC295 | pCL1920 harboring truncated pmrC295 | This study |
| pPmrC150lacZ′ | pPmrC150, fusion of pmrC150 and lacZ fragment | This study |
| pPmrC150phoA′ | pPmrC150, fusion of pmrC150 and phoA fragment | This study |
| pPmrC181lacZ′ | pPmrC181, fusion of pmrC181 and lacZ fragment | This study |
| pPmrC181phoA′ | pPmrC181, fusion of pmrC181 and phoA fragment | This study |
| pPmrC295lacZ′ | pPmrC181, fusion of pmrC295 and lacZ fragment | This study |
| pPmrC295phoA′ | pPmrC181, fusion of pmrC295 and phoA fragment | This study |
a
FRT, FLP recognition target.