Skip to main content
. 2014 Aug 1;35(11):2457–2466. doi: 10.1093/carcin/bgu159

Fig. 5.

Fig. 5.

ROS-mediated suppression of lysosomal degradation in AR cells. (A) and (B) A549 TR cells were treated with cycloheximide (CHX, 10 µM) for the indicated time periods. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified by densitometry (Image J) and normalized to the corresponding input control bands. MUC1 expression changes were calculated with the control taken as 100%. (C) A549 TR cells were treated with CQ (20 µM) and MG132 (10 µM) for 24h. MUC1 expression was detected by Western blot. β-Actin was detected as a loading control. (D) and (E) H460 TR cells were treated with CHX (10 µM) with or without BHA (100 μM) for the indicated time periods. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified by densitometry (Image J) and normalized to the corresponding input control bands. MUC1 expression changes were calculated with the control taken as 100%.