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. 2014 Sep 1;35(11):2436–2446. doi: 10.1093/carcin/bgu181

Fig. 3.

Fig. 3.

Quantitative PCR validations of expression arrays in MCF-7 and ZR-75-1 cell lines. Columns in each of the graphs are presented in the following order: control, siMBD2, 5 µM 5-azaCdR and Combined. (A) EMT markers, (B) pro-metastatic genes and (C) tumor suppressor genes. (D) Important biological functions. GAPDH was used as a reference gene in all cases. Values presented as mean ± SEM of triplicate experiments (*P < 0.05, two-tailed Student’s t-test). (E) siMbd2 seq 2 depletion (90nM): Expression profiles of uPA, MMP2 and MAEL were measured by quantitative PCR. (F) Correlation between Mbd2 normalized expression levels in ZR-75-1 in the different individual control and treatment samples and relative expression of Vimentin, FABP7, HAS3, SPARC and uPA (Axe X) in the same samples as determined by linear regression. GAPDH was used as a reference gene for normalization.