The sequence provided the upstream and downstream regions of bla
KPC-15 structural genes. The tnpA gene, which was upstream of the bla
KPC-15 (3,020 bp) gene, was homologous to a putative ISKpn8 transposon, and the downstream region of the bla
KPC-15 gene (1,320 bp) was homologous to a putative ISKpn6-like transposon. The nucleotides upstream of the bla
KPC-15 and bla
TEM-12 gene translational start codons were shown in the box. The putative −10 promoter elements of the bla
KPC-15 gene were shown as gattaa, labeled as −10 below, and there were no obvious −35 promoter elements to be discovered in the promoter region. The putative −10 and −35 promoter elements of the bla
TEM-12 gene were shown as tataac and ttattg, labeled as −10 and −35 below the promoter region. The start sites of transcription were indicated as G by +1 residue. RBS was the abbreviation of the ribosome binding site.