. 2004 Jul;186(13):4085–4099. doi: 10.1128/JB.186.13.4085-4099.2004

TABLE 1.

Strains and plasmids used in this study

Strain or plasmid	Relevant genotype and phenotype^a	Reference or source
Strains
E. coli XL1-Blue	recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacl^q ZΔM15 Tn10(Tc^r)]	Stratagene
S. aureus
BB255	rsbU, low σ^B activity	2
COL	mec, highly Mc^r clinical isolate, Mc^r Tc^r	41
Newman	Clinical isolate, high level of clumping factor (ATCC 25904)	17
IK181	BB255 ΔrsbUVW-sigB, Em^r	44
IK183	COL ΔrsbUVW-sigB, Em^r Mc^r Tc^r	44
IK184	Newman ΔrsbUVW-sigB, Em^r	44
GP268	BB255 rsbU⁺, Tc^r	26
Plasmids
pAC7	Cm^r, expression plasmid containing the P_BAD promoter and the araC gene	70
pAC7-sigB	Cm^r, 767-bp PCR fragment of the sigB ORF from strain COL into pAC7	This study
pSB40N	Ap^r, promoter probe plasmid	39
pSA0455p	Ap^r, 360-bp PCR fragment covering the promoter region of the COL homologue of ORF N315-SA0455 into pSB40N	This study

Abbreviations are as follows: Ap^r, ampicillin resistant; Cm^r, chloramphenicol resistant; Em^r, crythromycin resistant; Mc^r, methicillin resistant; Tc^r, tetracycline resistant.