TABLE 3.
Genes downregulated by σB
| N315 ORF no.a | N315 genea | N315 descriptiona | Fold changeb in strain:
|
Reference(s) with reported σB dependencec | Regulation by SarAd (reference[s]) | ||
|---|---|---|---|---|---|---|---|
| COL | Newman | GP268 | |||||
| N315-SA2430 | aur | Zinc metalloprotease aureolysin | 7.4 | 6.1 | 9.1 | 36, 84 | Down (16, 37, 84) |
| N315-SA2411 | citM | HP, similar to magnesium citrate secondary transporter | Down | Down | 4.3 | ||
| N315-SA0820 | glpQ | Glycerophosphoryl diester phosphodiesterase | 3.6 | 2.6 | 1.9 | 4 | Down (84) |
| N315-SA1007 | hla | α-Hemolysin precursor | 2.1 | 2.8 | 4.1 | 26, 84 | Up (16) |
| N315-SA2207 | hlgA | γ-Hemolysin component A | 1.7 | 2.0 | 2.1 | ||
| N315-SA2209 | hlgB | γ-Hemolysin component B | 2.2 | 4.2 | Down | Up (16) | |
| N315-SA2208 | hlgC | γ-Hemolysin component C | 2.0 | 4.7 | 4.1 | Up (16) | |
| N315-SA2463 | lip | Triacylglycerol lipase precursor | 2.0 | 6.2 | 2.0 | 44, 84 | Up (84)/Down (16) |
| N315-SA0252 | lrgA | Holin-like protein LrgA | 5.8 | 9.4 | Up (22) | ||
| N315-SA0253 | lrgB | Holin-like protein LrgB | 6.2 | 6.5 | Up (22)/Down (16) | ||
| N315-SA1812 | lukF | HP, similar to synergohymenotropic toxin precursor | 2.7 | 3.9 | Down | ||
| N315-SA1813 | lukM | HP, similar to leukocidin chain lukM precursor | 3.8 | 4.8 | Down | ||
| N315-SA0746 | nuc | Staphylococcal nuclease | 29.7 | 5.1 | Down | 44, 84 | Down (16, 84) |
| N315-SA0091 | plc | l-Phosphatidylinositol phosphodiesterase precurosr | Down | 3.9 | Down | Down (84) | |
| N315-SA0963 | pycA | Pyruvate carboxylase | 2.3 | 1.9 | 2.3 | ||
| N315-SA0259 | rbsD | Ribose permease | 2.9 | 2.8 | 1.5 | ||
| N315-SA0258 | rbsK | Probable ribokinase | 2.8 | 2.3 | 1.3 | ||
| N315-SA1758 | sak | Staphylokinase precursor (protease III) | 2.7 | 7.0 | |||
| N315-SA0128 | sodM | Superoxide dismutase | 4.6 | 2.0 | 2.8 | ||
| N315-SA1631 | splA | Serine protease SplA | Down | 9.9 | Down | 84 | Up (16) |
| N315-SA1630 | splB | Serine protease SplB | Down | 7.9 | Down | Up (16) | |
| N315-SA1629 | splC | Serine protease SplC | Down | Down | Down | 84 | |
| N315-SA1628 | splD | Serine protease SplD | Down | Down | Down | Up (16) | |
| COL-SA1865 | splE | Serine protease SplE | Down | Down | Down | ||
| BAB95617_1 | splF | Serine protease SplF | Down | Down | |||
| N315-SA0901 | sspA | Staphylococcal serine protease (V8 protease) | 3.8 | 2.1 | 3.3 | 36, 84 | Down (36) |
| N315-SA0900 | sspB | Cysteine protease | 3.2 | 2.2 | 4.3 | 36 | Down (16, 36) |
| N315-SA0899 | sspC | Cysteine protease | 3.0 | 1.9 | 3.0 | Down (16) | |
| N315-SA2302 | stpC | HP, similar to ABC transporter | 6.3 | 2.3 | 4.0 | ||
| N315-SA0022 | HP, similar to 5′ nucleotidase | 2.6 | 1.8 | 3.3 | |||
| N315-SA0089 | HP, similar to DNA helicase | 2.4 | Down | 2.1 | |||
| N315-SA0260 | HP, similar to ribose transporter RbsU | 3.0 | 2.6 | 2.3 | |||
| N315-SA0270 | HP, similar to secretory antigen precursor SsaA | 4.6 | Down | Down | |||
| N315-SA0272 | HP, similar to transmembrane protein Tmp7 | 4.4 | Down | Down | |||
| N315-SA0276 | Conserved HP, similar to diarrheal toxin-like protein | 3.7 | Up | ||||
| N315-SA0285 | HP | 2.6 | Down | 3.4 | |||
| N315-SA0291 | HP | 3.1 | 3.3 | ||||
| N315-SA0295 | HP, similar to outer membrane protein precursor | 4.9 | 3.6 | 10.4 | |||
| N315-SA0368 | HP, similar to proton/sodium glutamate symport protein | 2.7 | 3.1 | 1.4 | |||
| N315-SA0841 | HP, similar to cell surface protein Map-w | 5.7 | 3.4 | 2.2 | |||
| N315-SA0977 | 29-kDa cell surface protein | 2.5 | 2.1 | 1.8 | |||
| N315-SA1725 | Staphopain, cysteine protease | 5.9 | 4.2 | 10.6 | 36, 84 | Down (36, 84) | |
| N315-SA1726 | HP | 3.8 | 3.4 | 6.5 | |||
| N315-SA1815 | HP, similar to Na+-transporting ATP synthase | Down | Down | Down | |||
| N315-SA1853 | HP, similar to DNA mismatch repair protein MutS | 2.1 | Down | 4.0 | |||
| N315-SA2132 | HP, similar to ABC transporter (ATP-binding protein) | 2.7 | Down | 2.3 | |||
| N315-SA2133 | Conserved HP | 3.1 | Down | 3.2 | |||
| N315-SA2303 | HP, similar to membrane-spanning protein | Down | 1.8 | Down | |||
| N315-SAS020 | HP, similar to phosphoglycerate mutase | 2.1 | 2.4 | 2.2 | |||
| COL-SA0450 | HP | 2.2 | 2.2 | 3.1 | |||
| COL-SA1884 | HP | 3.3 | Down | Down | |||
| COL-SA2693 | HP | 2.2 | 7.1 | 2.2 | |||
a
Based on the published sequence of strain N315 (accession no. NC_002745). For genes not present in N315, the gene name and description given are from the COL genome, available from The Institute for Genomic Research Comprehensive Microbial Resource website (http://www.tigr.org) or the respective accession number. HP, hypothetical protein.
b
Normalized values in the ΔrsbUVW-sigB mutant over values in the rsbU+V+W+ sigB+ strain. “Down” denotes genes highly downregulated in the rsbU+V+W+ sigB strain, such that the transcripts were below detectable levels and the change could not be accurately calculated.
c
References reporting an influence of σB on the respective gene or its gene product.
d
References reporting an influence of SarA on the respective gene or its gene product.