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. 2014 Oct 31;9(10):e111611. doi: 10.1371/journal.pone.0111611

Figure 6. CDK2 is involved in regulating CDKN3-mediated leukemic cell survival.

Figure 6

(A) Shown is lentiviral vectors constructed in this study that encode luciferase shRNA (sh-luc) control, CDKN3 shRNA (sh-CDKN3), sh-CDKN3 and either wild type CDK2 (CDK2-WT) or CDK2 dominant-negative mutant (CDK2-D145N). (B) RT-PCR and Western blotting were performed to examine the expression of CDKN3, CDK2, and XIAP in K562 cells expressing sh-CDKN3 alone, sh-CDKN3 and CDK2-WT, sh-CDKN3 and CDK2-D145N, or control. (C) Survival of K562 cells expressing sh-CDKN3, sh-CDKN3 and CDK2-WT, sh-CDKN3 and CDK2-D145N, or the control was analyzed by flow cytometry after treatment with or without 10 µM of imatinib for 48 h. Plotted are results from three independent experiments. Error bars represent SEM, n = 3; *P<0.05.