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(A) Anterior pituitary sections and (B) cultured anterior pituitary cells from OVX rats were processed for identification of HNr and pituitary hormones by double immunofluorescence. Left panels: nuclear staining with DAPI; middle panels: immunocytochemistry for HNr; right panels: immunocytochemistry for prolactin (PRL) or growth hormone (GH). Arrows show a lactotrope and a somatotrope expressing HNr. Scale bars: 10 µm.
