Figure 4. Estradiol decreases HNr expression in anterior pituitary cells from OVX female rats.

Cultured anterior pituitary cells from OVX rats were incubated with 17β-estradiol (E₂, 10⁻⁹ M) or vehicle (Veh, ethanol 1 µl/l) for 24 h, immunostained for HNr and analyzed by flow cytometry. Each column represents the mean ± SE of (A) the percentage of HNr-positive cells, and (B) the fluorescence intensity of HNr staining (Gmean) from 3 independent experiments (3 replicates each one). The upper panels show representative dot plots and histograms of HNr expression in anterior pituitary cells from OVX rats incubated with 17β-estradiol. *p<0.05, Student’s t test.