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. 2014 Oct 31;9(10):e111548. doi: 10.1371/journal.pone.0111548

Figure 5. Estradiol decreases HNr expression in anterior pituitary cells from GNX male rats.

Figure 5

Cultured anterior pituitary cells from GNX male rats were incubated with (A) dihydrotestosterone (DHT, 10−8 M) or vehicle (Veh, ethanol 10 µl/l) or (B) 17β-estradiol (E2, 10−9 M) or vehicle (Veh, ethanol 1 µl/l) for 24 h, immunostained for HNr and analyzed by flow cytometry. Each column represents the mean ± SE of the percentage of HNr-positive cells from 3 independent experiments (3 replicates each one). The upper panels show representative dot plots of HNr expression in anterior pituitary cells from GNX rats incubated with dihydrotestosterone or 17β-estradiol. *p<0.05, Student’s t test.