Figure 8. HN protects GH3 cells from TNF-α-induced apoptosis.

Cultured GH3 cells were incubated with HN (5 µM) for 2 h before adding TNF-α (50 ng/ml) for 24 h. Apoptosis was assessed by the TUNEL method. Each column represents the percentage ± CL of TUNEL-positive cells (n≥1000 cells/group). Data from two separate experiments were analyzed by χ² test. **p<0.01 respective control without TNF-α, ∧∧p<0.01 vs respective control without HN.