. 2004 Jul;186(13):4295–4306. doi: 10.1128/JB.186.13.4295-4306.2004

TABLE 1.

Oligonucleotide primers used for PCR and sequencing

Gene	Primer	Primer sequence (5′ to 3′)	Gene location	Amplicon Size (bp)
ompA	Nlo	ATGAAAAAACTCTTGAAATCG	1 to 21^a	1,129
	Nro	CTCAACTGTAACTGCGTATTT	1128 to 1108^a
ompA	Pctm3	TCCTTGCAAGCTCTGCCTGTGGGGAATCCT	55 to 84^a	1,014
	Sero2A	TTTCTAGATTTCATTTTGTT	1068 to 1049^a
ompA	OmpA-3^b	CCTGCTGAACCAAGCCTTATG	82 to 102^a
ompA	OmpA-4^b	CCAATATGCTCAATCTAAACC	639 to 659^a
pmpC	C-9^b	GCTGGTCAAGTTATCTGCGGAGTG	−344 to −321^d	3,015
	C-10^b	TATTCCCGGAGAAGGTGACAGTTC	2671 to 2648^c
pmpC	C-11^b	TGGAGATAGCGCTGGAGACTCTGA	2394 to 2417^c	3,115
	C-12^b	GTTAACGCGTACCGAGGGTTCG
pmpC	C-7^b	AGACAACACAGAGTATCGAG	153 to 172^c
pmpC	C-5^b	ATAACTACTCCCCCTCTCATAGGA	1156 to 1179^c
pmpC	C-4^b	GGGGGTAGAAGATTCTGGGGTATC	1362 to 1339^c
pmpC	C-8^b	CTTATCCCACTATAACTCTG	1713 to 1732^c
pmpC	C-15^b	ACTCTCCTACTGTAACCATTG	2720 to 2740^c
pmpC	C-17^b	AACAGGTACACAGGCAACTG	3450 to 3469^c
pmpC	C-16^b	TGACGGATACTGGAGTATTC	4940 to 4921^c
pmpH	H-1^b	TCGGGCATTCAGGAGTGGACAT	−367 to −346^e	776
	H-2	CTTCGCCTGCTCCGGAAATACTC	408 to 386^e
pmpI	I-1	GAGGCGATTCTTTGCTGCTACTT	1095 to 1117^f	1,369
	I-2^b	AACAAACCAACCCAAAACTAAAAT	−273 to −250^f

Primers designed based on the sequence of strain L₂ ompA.

Primers used for automated sequencing.

Primers designed based on the sequence of strain D pmpC.

−, a region upstream from the start codon.

Primers designed based on the sequence of strain D pmpH.

Primers designed based on the sequence of strain D pmpI.