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. 2014 Oct 31;9(10):e111536. doi: 10.1371/journal.pone.0111536

Figure 5. MLA inhibits Aβ-induced autophagy process in SH-SY5Y cells.

Figure 5

(A) Western blot analysis of the protein level of LC3 in SH-SY5Y cells treated with 10 µM Aβ25–35 with or without MLA. (B) Immunofluorescence microscopy of punctate pattern of LC3 localization in SH-SY5Y cells treated with 10 µM Aβ25–35 with or without MLA. (C) Fluorescence microscopy of the formation of acidic vesicles with MDC staining in SH-SY5Y cells treated with 10 µM Aβ25–35 with or without MLA. (D) Flow cytometry of the formation of acidic vesicles after MDC staining in SH-SY5Y cells treated with 10 µM Aβ25–35 with or without MLA.