Figure 5. Inhibition of anchorage-independent growth of A549 and AGS cancer cells by the acetone extract of F. cucullata and usnic acid in sub-lethal concentrations.

(A–B) Clonogenic assay of A549 and AGS cells treated with the F. cucullata extract, usnic acid, or lichesterinic acid (A) and quantificational analysis of colony number in each group (B). (C–D) Soft agar colony-formation assay of A549 and AGS cells treated with F. cucullata extract, usnic acid, or lichesterinic acid (C) and quantificational analysis of percent colony area in each group (D). Representative images are shown from three independent experiments. Data represent mean ± S.E.M. (standard error of the mean), n = 3. *p<0.05; **p<0.01; ***p<0.001; NS, no significant difference compared to the dimethylsulfoxide-treated group.