TABLE 2.
Seventeen novel F. diplosiphon genes regulated by light color
Light response category and gene or groupa | Subarray result
|
Product size (bp) | Avg RL intensity | Avg GL intensity | Northern blotting result
|
tc | nd | ||
---|---|---|---|---|---|---|---|---|---|
FIb | SE | FIb | SE | ||||||
GL | |||||||||
3088E12-C | 1.56 | 0.09 | 357 | 1,843 | 3,128 | 1.76 | 0.12 | 3.60 | 6 |
3091A7-A | 1.24 | 0.05 | 1,641 | 9,036 | 11,525 | 1.63 | 0.07 | 5.58 | 3 |
3104G9-B | 1.79 | 0.04 | 338 | 3,009 | 5,688 | 1.39 | 0.11 | 2.59 | 5 |
3109A9-C | 1.32 | 0.03 | 806 | 4,630 | 5,995 | 2.24 | 0.07 | 7.59 | 6 |
Group A-C | 1.36 | 0.05 | 309 | 16,584 | 22,219 | 2.00 | 0.08 | 5.83 | 5 |
Group A-D | 1.33 | 0.04 | 267 | 9,789 | 13,046 | 1.92 | 0.09 | 9.55 | 4 |
Group C-E | 1.71 | 0.04 | 849 | 11,324 | 17,906 | 2.18 | 0.11 | 5.10 | 5 |
Group E-A | 2.66 | 0.04 | 553 | 3,884 | 12,440 | 6.54 | 0.04 | 23.87 | 6 |
Group E-B | 2.05 | 0.06 | 271 | 1,495 | 2,896 | 5.81 | 0.05 | 16.79 | 6 |
Group E-G | #e | 7.52 | 0.05 | 19.39 | 5 | ||||
cpeBA | 11.4 | 0.02 | 1,100 | 6,103 | 56,877 | —f | |||
cpeCDE | 5.127 | 0.05 | 3,500 | 10,662 | 54,176 | — | |||
cpeSTR | 2.77 | 0.05 | 3,000 | 8,591 | 16,735 | — | |||
RL | |||||||||
3091A7-B | 1.33 | 0.10 | 605 | 1,816 | 1,534 | 2.09 | 0.41 | 2.67 | 4 |
3092A9-C | 1.27 | 0.07 | 1,591 | 2,908 | 2,524 | 2.27 | 0.58 | 2.19 | 4 |
3098H5-C | 1.30 | 0.12 | 497 | 1,626 | 1,426 | 1.74 | 0.36 | 2.07 | 6 |
3105B10-A | 1.55 | 0.18 | 1,595 | 1,621 | 1,237 | 2.05 | 0.09 | 11.72 | 4 |
3106A6-A | 1.27 | 0.05 | 2,020 | 6,715 | 5,971 | 1.91 | 0.33 | 2.77 | 4 |
3106F10-A | 1.37 | 0.10 | 1,932 | 2,445 | 2,221 | 1.98 | 0.31 | 3.14 | 4 |
3106F10-B | 1.33 | 0.23 | 2,794 | 1,793 | 1,688 | 1.54 | 0.23 | 2.34 | 5 |
cpc2 | 11.99 | 1.95 | 1,400 | 59,661 | 6,511 | — | |||
Nonresponsive | |||||||||
apcAB | 0.89 | 0.02 | 600 | 4,932 | 6,132 | — | |||
cpc1 | 0.98 | 0.10 | 3,500 | 4,949 | 5,280 | — |
Control genes cpeBA, cpeCDE, cpc1, cpc2, and apcAB were included for comparison.
FI (fold induction) values were determined by dividing RL intensity by GL intensity for RL-induced genes and GL intensity by RL intensity for GL-induced genes. For control genes apcAB and cpc1, FI values are given as RL intensity divided by GL intensity.
t, absolute value from a Student's one-tailed t test.
n, number of biological replicates for the Northern blot analysis.
#, ORF group E-G is a homologue of Nb1A and was not printed on the subarrays.
—, control genes were not analyzed by Northern blotting in this series of experiments. See reference 57 for details of their expression characteristics.