TABLE 2.
Seventeen novel F. diplosiphon genes regulated by light color
| Light response category and gene or groupa | Subarray result
|
Product size (bp) | Avg RL intensity | Avg GL intensity | Northern blotting result
|
tc | nd | ||
|---|---|---|---|---|---|---|---|---|---|
| FIb | SE | FIb | SE | ||||||
| GL | |||||||||
| 3088E12-C | 1.56 | 0.09 | 357 | 1,843 | 3,128 | 1.76 | 0.12 | 3.60 | 6 |
| 3091A7-A | 1.24 | 0.05 | 1,641 | 9,036 | 11,525 | 1.63 | 0.07 | 5.58 | 3 |
| 3104G9-B | 1.79 | 0.04 | 338 | 3,009 | 5,688 | 1.39 | 0.11 | 2.59 | 5 |
| 3109A9-C | 1.32 | 0.03 | 806 | 4,630 | 5,995 | 2.24 | 0.07 | 7.59 | 6 |
| Group A-C | 1.36 | 0.05 | 309 | 16,584 | 22,219 | 2.00 | 0.08 | 5.83 | 5 |
| Group A-D | 1.33 | 0.04 | 267 | 9,789 | 13,046 | 1.92 | 0.09 | 9.55 | 4 |
| Group C-E | 1.71 | 0.04 | 849 | 11,324 | 17,906 | 2.18 | 0.11 | 5.10 | 5 |
| Group E-A | 2.66 | 0.04 | 553 | 3,884 | 12,440 | 6.54 | 0.04 | 23.87 | 6 |
| Group E-B | 2.05 | 0.06 | 271 | 1,495 | 2,896 | 5.81 | 0.05 | 16.79 | 6 |
| Group E-G | #e | 7.52 | 0.05 | 19.39 | 5 | ||||
| cpeBA | 11.4 | 0.02 | 1,100 | 6,103 | 56,877 | —f | |||
| cpeCDE | 5.127 | 0.05 | 3,500 | 10,662 | 54,176 | — | |||
| cpeSTR | 2.77 | 0.05 | 3,000 | 8,591 | 16,735 | — | |||
| RL | |||||||||
| 3091A7-B | 1.33 | 0.10 | 605 | 1,816 | 1,534 | 2.09 | 0.41 | 2.67 | 4 |
| 3092A9-C | 1.27 | 0.07 | 1,591 | 2,908 | 2,524 | 2.27 | 0.58 | 2.19 | 4 |
| 3098H5-C | 1.30 | 0.12 | 497 | 1,626 | 1,426 | 1.74 | 0.36 | 2.07 | 6 |
| 3105B10-A | 1.55 | 0.18 | 1,595 | 1,621 | 1,237 | 2.05 | 0.09 | 11.72 | 4 |
| 3106A6-A | 1.27 | 0.05 | 2,020 | 6,715 | 5,971 | 1.91 | 0.33 | 2.77 | 4 |
| 3106F10-A | 1.37 | 0.10 | 1,932 | 2,445 | 2,221 | 1.98 | 0.31 | 3.14 | 4 |
| 3106F10-B | 1.33 | 0.23 | 2,794 | 1,793 | 1,688 | 1.54 | 0.23 | 2.34 | 5 |
| cpc2 | 11.99 | 1.95 | 1,400 | 59,661 | 6,511 | — | |||
| Nonresponsive | |||||||||
| apcAB | 0.89 | 0.02 | 600 | 4,932 | 6,132 | — | |||
| cpc1 | 0.98 | 0.10 | 3,500 | 4,949 | 5,280 | — | |||
a
Control genes cpeBA, cpeCDE, cpc1, cpc2, and apcAB were included for comparison.
b
FI (fold induction) values were determined by dividing RL intensity by GL intensity for RL-induced genes and GL intensity by RL intensity for GL-induced genes. For control genes apcAB and cpc1, FI values are given as RL intensity divided by GL intensity.
c
t, absolute value from a Student's one-tailed t test.
d
n, number of biological replicates for the Northern blot analysis.
e
#, ORF group E-G is a homologue of Nb1A and was not printed on the subarrays.
f
—, control genes were not analyzed by Northern blotting in this series of experiments. See reference 57 for details of their expression characteristics.