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PS1 can undergo endoproteolysis in the absence of Pen-2. (a) Western blot and (b) quantification showing a time course of Pen-2 KO MEF cell treatment with 10 μM MG132 or vehicle. (c) Western blot of microsomes prepared from Pen-2 KO MEF cells in the absence or presence of 10 μM pepstatin A. The left two lanes contained lysates of Pen-2 KO rescued with F-Pen-2 or without rescue.
