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Spectrofluorimetric measurement of the affinity of full-length factor XIa (A) and factor XIa–DEGR (B) for β-SPGG-2, UFH, and H8 at pH 7.4 and 37 °C using intrinsic tryptophan (A, λEM = 348 nm, λEX = 280 nm) or dansyl (B, λEM = 547 nm, λEX = 345 nm) fluorescence. Solid lines represent nonlinear regressional fits using quadratic eq 4. (C) Change in the fluorescence emission spectrum of DEGR-factor XIa (λEX = 345 nm) induced by the interaction with β-SPGG-2 at pH 7.4 and 37 °C.
