Table 4. Salt Dependence of Affinity Studies for β-SPGG-2, UFH, and H8 at pH 7.4 and 37 °C.
| slopea | Za | intercepta | KD,NI (μM) | ΔG0NI (kcal/mol) | ΔG0NI (%)b | |
|---|---|---|---|---|---|---|
| β-SPGG-2 | 0.71 ± 0.13c | 0.87 ± 0.16 | –5.77 ± 0.16 | 1.7 ± 0.3 | 8.2 ± 0.1 | 88.6 |
| UFH | 0.73 ± 0.20 | 0.89 ± 0.24 | –5.14 ± 0.25 | 7.2 ± 0.3 | 7.3 ± 0.03 | 87.4 |
| H8 | 0.52 ± 0.03 | 0.64 ± 0.04 | –5.00 ± 0.04 | 10.1 ± 0.4 | 7.1 ± 0.02 | 90.5 |
a
Slope, Z, and intercept were calculated from linear regressional analysis of log KD,obs versus log[Na] as defined by eq 4.
b
Nonionic binding energy contribution to the total is expressed as percentage.
c
Error represent standard error calculated using global fit of the data.