TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotypea | Reference or source |
|---|---|---|
| S. meliloti | ||
| Rm1021 | Strr SU47 | 26 |
| DKR227 | lpsS::pDW33 | This study |
| DKR229 | lpsS::pDW33/pMB393 | This study |
| DKR230 | lpsS::pDW33/pMB393::lpsS | This study |
| DKR118 | nodP1Q1::Tn5-233 nodP2Q2::Tn5 | This study |
| DKR62 | lpsL::pVO155 | 18 |
| E. coli | ||
| BL21 (DE3) | T7-expressing strain | 40, 41 |
| Plasmid | ||
| pMB393 | Broad-host-range plasmid | 1 |
| pDKR161 | pMB393::lpsS | This study |
| pET16B | T7 expression vector | 40, 41 |
| pCR2.1 | Topo cloning vector | Invitrogen |
| pDKR252 | pCR2.1::lpsS-His6 | This study |
| pDW33 | Insertional inactivation plasmid; Hygr | Wells and Long, unpublished |
| pDKR227 | pDW33::lpsS (internal fragment) | This study |
a
All strains are derived from strain Rm1021.