TABLE 3.
Expression of lpsS::uidA fusion during log-phase and stationary-phase growtha
Strain | Fusion | GUS activity during:
|
||
---|---|---|---|---|
Log phaseb | Entry into stationary phasec | Late stationary phased | ||
Rm1021 | None (background) | 8.1 | ND | 7.5 |
DKR227 | lpsS::uidA | 3,407 ± 247 | 3,138 ± 112 | 3,044 ± 55 |
DKR50e | SMa21243::uidA | 91.5 ± 2.5 | 119.9 ± 7 | 403.2 ± 21.2 |
Activity is in Miller units (27). GUS, β-glucuronidase.
Log phase corresponds to an OD600 of 0.5.
Entry into stationary phase corresponds to an OD600 of 1.5. ND, not done.
Late stationary log phase corresponds to an OD600 of 2.5.
DKR50 contains a fusion to an ORF (SMb21243) previously shown to be up-regulated upon entry into stationary phase (45).