TABLE 1.
Strains and plasmids used in this work
| Strain or plasmid | Description | Reference |
|---|---|---|
| Strains | ||
| MC4100 | F−araD139 Δ(argF-lac)U169 rpsL150 (Strr) relA1 flbB5301 deoC1 ptsF25 rbsR | 7 |
| TR50 | MC4100 λRS88 [cpxP′-lacZ+] | 48 |
| MC1061 | F−araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (Strr) hsdR2(rK− mK+) mcrA mcrB1 | 63 |
| Plasmids | ||
| pMMB66 | Expression vector | 16 |
| pPAP5 | Wild-type pap operon | 36 |
| pWT | PapD expression plasmid | 23 |
| pTRC-G1 | papG1 | K. Dodson, unpublished data |
| pFJ7 | papF | 27 |
| pHJ13 | Wild-type PapE expression plasmid | 28 |
| pFJ11 | papK | 27 |
| pFGS2 | N-terminally deleted papE | 51 |
| pYML33 | DscEPapE expression plasmid | This study |
| pYML34 | DscKPapE expression plasmid | This study |
| pYML36 | NtdPapE expression plasmid | This study |
| pYML42 | Wild-type PapG1 expression plasmid | This study |
| pYML43 | Wild-type PapF expression plasmid | This study |
| pYML44 | Wild-type PapK expression plasmid | This study |
| pYML50 | Wild-type PapA expression plasmid | This study |
| pYML55 | NtsEPapF expression plasmid | This study |
| pYML56 | NtsFPapE expression plasmid | This study |
| pYML64 | NtdPapF expression plasmid | This study |
| pYML75 | NtsEPapK expression plasmid | This study |
| pYML80 | NtsEPapA expression plasmid | This study |
| pYML83 | NtsKPapE expression plasmid | This study |
| pYML87 | NtsAPapE expression plasmid | This study |