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. 2004 Jul;186(13):4307–4314. doi: 10.1128/JB.186.13.4307-4314.2004

FIG. 2.

FIG. 2.

Nucleotide sequence alignment around the I-HmuII cleavage site. The sequence of the top (coding) strand is shown, with the cleavage site of I-HmuII on the bottom strand indicated by an arrow. Differences from the SP82 sequence are highlighted. Oligonucleotide SP82c1 has eight mismatches to the SPO1 sequence, four on each side of the cleavage site, requiring bidirectional conversion to hybridize with SPO1 DNA.