TABLE 2.
SPO1 intron homing is a gene conversion event
Phage cross | Host | No. | Distribution of genetic markers in progenya
|
|||||||
---|---|---|---|---|---|---|---|---|---|---|
sus14,I+ | wt14,ΔI | wt14,I+ | sus14,ΔI | I+ | ΔI | sus14 | wt14 | |||
SPO1sus14 × SPO1ΔI | None | 1 | 54 | 46 | 0 | 0 | 54 | 46 | 54 | 46 |
2 | 41 | 59 | 0 | 0 | 41 | 59 | 41 | 59 | ||
3 | 34 | 66 | 0 | 0 | 34 | 66 | 34 | 66 | ||
Sup− | 1 | 52 | 15 | 32 | 1 | 84 | 16 | 53 | 47 | |
2 | 49 | 10 | 40 | 1 | 89 | 11 | 50 | 50 | ||
3 | 44 | 5 | 51 | 0 | 95 | 5 | 56 | 44 | ||
Sup+ | 1 | 58 | 3 | 33 | 0 | 91 | 8 | 58 | 41 | |
2 | 52 | 3 | 45 | 0 | 97 | 3 | 52 | 48 | ||
3 | 43 | 4 | 53 | 0 | 96 | 4 | 43 | 57 | ||
SPO1(sus14,I-HmuIoc) × SPO1ΔI | None | 1 | 47 | 53 | 0 | 0 | 47 | 53 | 47 | 53 |
2 | 35 | 65 | 0 | 0 | 35 | 65 | 35 | 65 | ||
Sup− | 1 | 28 | 53 | 11 | 6 | 37 | 59 | 36 | 64 | |
2 | 41 | 47 | 5 | 7 | 46 | 54 | 48 | 52 | ||
Sup+ | 1 | 53 | 7 | 40 | 0 | 93 | 7 | 53 | 47 | |
2 | 47 | 6 | 46 | 1 | 93 | 7 | 48 | 52 | ||
SPO1 × SPO1(sus14,ΔI) | None | 1 | 0 | 0 | 65 | 35 | 65 | 35 | 35 | 65 |
2 | 0 | 0 | 48 | 52 | 48 | 52 | 52 | 48 | ||
Sup− | 1 | 31 | 0 | 69 | 0 | 100 | 0 | 31 | 69 | |
2 | 33 | 0 | 65 | 2 | 98 | 2 | 35 | 65 | ||
Sup+ | 1 | 30 | 2 | 65 | 3 | 95 | 5 | 33 | 67 | |
2 | 32 | 0 | 65 | 3 | 97 | 3 | 35 | 65 | ||
SPO1I-HmuIoc × SPO1(sus14,ΔI) | None | 1 | 0 | 0 | 69 | 31 | 69 | 31 | 69 | 31 |
2 | 0 | 0 | 55 | 45 | 55 | 45 | 55 | 45 | ||
Sup− | 1 | 8 | 6 | 50 | 36 | 58 | 42 | 56 | 44 | |
2 | 6 | 9 | 43 | 41 | 49 | 50 | 47 | 42 | ||
Sup+ | 1 | 34 | 0 | 62 | 4 | 96 | 4 | 62 | 38 | |
2 | 29 | 1 | 67 | 3 | 96 | 4 | 68 | 32 |
wt14 indicates wild-type gene 14. I+ and ΔI indicate the presence or absence of introns, respectively.