TABLE 1.
Oligonucleotides designed for cloning, sequencing, or expressing the ponA gene of E. faecalis JH2-2
| Name | Sequencec (5′-3′) | Position in AJ302065 sequence |
|---|---|---|
| EFV583Motif5a | GTCGTACATTGTGTAAAGTACAACATCCCGGCGTTC | 2505-2470 |
| EFV583UP1 | GAATGCGACAGTTTCATCGAAGCTTTACGb | 1932-1960 |
| EFV583Motif3a | ATGCTTCTTGTGCTTTCCGTTTCAAGGTTTGGT | 2245-2213 |
| EFV583Motif3R | GACCAAACCTTGAAACGGAAAGCACAAGAAGCATGG | 2212-2247 |
| EF-RP2a | CAATTCTTTGTGGCTTGTCAATTCCACCCAGb | 4244-4214 |
| EF-RP3a | GGTAAAACTTGTACATTTGGTACCTCATAGGCb | 3776-3745 |
| EF-RP4a | GTTAGAAGTCCCTGTTTTGGCCGCTTGGb | 3477-3449 |
| EF-RP5a | TTTATCCACCATGAGGCGACCTGTCb | 2979-2955 |
| EF-Tpase1 | CAATCATGGATTATGGTCCCGCAATTGAGAb | 2912-2941 |
| EF-ponAa | CGATGAGGTGTTGGATGCATGTCTAGCAGCb | 1767-1738 |
| pET-EcoRI | TGGTTCGAATTCCGACAAGCCCCAAAATTAGAAGATGAC | 1900-1926 |
| pET-NotI | GGTGAGCGGCCGCCTTATTATGCTGCTTTATTTTC | 4047-4029 |
a
Oligonucleotide on the complementary strand.
b
Cy5 primer.
c
Underlined sequences are EcoRI and NotI restriction sites.