TABLE 2.
PBPa |
kcat/Km (M−1 s−1) for:
|
Reference or source | |
---|---|---|---|
Bz-D-Ala-Thg | Bz-Gly-Thg | ||
Class A | |||
His*PBP1 (E. faecalis) | 230 ± 15 | 430 ± 20 | This work |
PBP1a* (S. pneumoniae R6) | 128 | 5.7 | 8 |
PBP2a* (S. pneumoniae R6) | 220 | NDb | 9 |
PBP1* (M. leprae) | 4,500 | ND | 26 |
Class B | |||
PBP2x* (S. pneumoniae R6) | 3,600 | 160 | 43 |
t-PBP3s* (E. hirae) | 3,200 | <20 | 1 |
PBP2b* (S. pneumoniae) | 300 | <20 | 1 |
PBP3* (E. coli) | 100 | 20 | 2 |
LMM | |||
PBP R61 (Streptomyces sp. strain R61) | 100,000 | 430,000 | 7 |
PBP R39 (Actinomadura sp. strain R39) | 10,000 | 336,000 | 7 |
*, soluble derivative of the wild-type protein; t-PBP3s, tryptic derivative of PBP3s.
ND, not determined.