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. 2004 Jul;186(13):4412–4416. doi: 10.1128/JB.186.13.4412-4416.2004

TABLE 2.

Hydroysis parameters of thiolester substrates for different PBPs

PBPa kcat/Km (M−1 s−1) for:
Reference or source
Bz-D-Ala-Thg Bz-Gly-Thg
Class A
    His*PBP1 (E. faecalis) 230 ± 15 430 ± 20 This work
    PBP1a* (S. pneumoniae R6) 128 5.7 8
    PBP2a* (S. pneumoniae R6) 220 NDb 9
    PBP1* (M. leprae) 4,500 ND 26
Class B
    PBP2x* (S. pneumoniae R6) 3,600 160 43
    t-PBP3s* (E. hirae) 3,200 <20 1
    PBP2b* (S. pneumoniae) 300 <20 1
    PBP3* (E. coli) 100 20 2
LMM
    PBP R61 (Streptomyces sp. strain R61) 100,000 430,000 7
    PBP R39 (Actinomadura sp. strain R39) 10,000 336,000 7
a

*, soluble derivative of the wild-type protein; t-PBP3s, tryptic derivative of PBP3s.

b

ND, not determined.