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Circulating tumour cells (CTCs) from a patient (B1) with bladder cancer. A: The CTCs immediately after the separations. B: The CTCs on the separation membrane grown in vitro for 14 days creating a cell layer. C: The CTCs growing on the membrane. D: The CTCs growing on the membrane counterstained by CK-7 antibody (DAB-visualized). A bar represents 10 μm.
