FIG. 1.

Characterization of the mutated epitope and the mutant virus. (A) Biacore specific binding curves. Using BIAevaluation 3.1 software, sensorgram data from five separate concentrations of 3B3 MAb injected over bound wild-type (WT) epitope synthetic peptide (top five curves) or bound mutant (MT) synthetic peptide (bottom five curves) were merged onto a single timeline. (B) Concentrations of 3B3 MAb used in the experiment shown in panel A. To achieve specific binding, the binding from a control chip was subtracted from total binding shown by either the WT or MT synthetic peptide. The kinetic and equilibrium constants were determined using the Biacore technology as described in Materials and Methods. (C) Infectious center assay of WT and MT virus. Infectious center assays were carried out by previously described methods (8, 20). The numbers of infectious centers present at 24 and 48 h postinfection were determined for WT and MT viruses. The average increase of infectious centers at each interval was tabulated.