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. 2014 Oct 24;14:412. doi: 10.1186/1472-6882-14-412

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© Liu et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Hederagenin-induced apoptosis (A) and apoptotic morphological changes (B) in LoVo cells. a, control; b, 1 μΜ hederagenin; c, 2 μΜ hederagenin. LL(lower left), living cells (AV/PI negative); LR (lower right), early apoptotic cells (AV positive/PI negative); UR (upper right), late apoptotic cells (AV positive/PI positive); UL(upper left), necrotic cells(AV negative/PI positive). The numbers represent the percentage of the cells in the sum of upper right and lower right quadrants. For apoptotic morphological changes, apoptotic cells were detected by Hoechst 33342 staining and measured by fluorescence microscopy (magnification 200×).