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. 2004 Jul;78(13):7270–7273. doi: 10.1128/JVI.78.13.7270-7273.2004

FIG. 1.

FIG. 1.

Effects of postinfection RNAi on the abundance of JCV proteins in JCV-infected SVG-A cells. (a) Schematic representation of major early and late mRNAs of JCV. Major early mRNAs encode the small t antigen and T-Ag, which are translated as splicing variants. Two major forms of late mRNA encode either agnoprotein and VP1 or agnoprotein, VP2, and VP3. The regions of the viral RNAs targeted by the siRNAs are indicated by arrows. (b) Immunoblot analysis of the abundance of VP1 and agnoprotein of JCV in SVG-A cells at the indicated times after infection with JCV. (c) Schedule for JCV infection and siRNA transfection in SVG-A cells. (d) Immunoblot analysis of the indicated proteins in JCV-infected cells subjected to transfection with the indicated siRNAs. (e) Immunoblot analysis of the indicated proteins in JCV-infected cells subjected to transfection with the Ag122 siRNA at 60 or 120 pmol/well or with a lamin A/C-specific siRNA. Control (infected) cells were subjected to mock transfection.