Table 1.
Species | Read length | Raw reads | Clean reads | Assembler | N50 | Contig count |
---|---|---|---|---|---|---|
H. annuus | 101 | 2 x 21,404,702 | 40,742,686 | CLC (ws60,paired) | 482 | 59,530 |
A. montana | 101 | 2 x 14,458,043 | 27,516,042 | CLC (ws60,paired) | 485 | 45,194 |
Z. haageana | 101 | 2 x 38,382,090 | 64,649,107 | CLC (autows,non-paired) | 308 | 205,324 |
Z. haageana | 101 | 2 x 38,382,090 | 64,649,107 | CLC (ws60,paired) | 435 | 80,460 |
Z. haageana | 101 | 2 x 38,382,090 | 72,756,408 | CLC (ws60,paired) | 629 | 40,764 |
H. helianthoides | 101 | 2 x 109,627,594 | 169,128,716 | CLC (autows,non-paired) | 305 | 443,800 |
H. helianthoides | 101 | 2 x 109,627,594 | 169,128,716 | CLC (ws60,paired) | 497 | 151,272 |
H. helianthoides | 101 | 2 x 109,627,594 | 200,130,791 | CLC (ws60,paired) | 496 | 162,563 |
Clean reads were assembled using two methods; automatic word size (autows, 23), non-paired and word size 60 (ws60), paired method. Number of clean reads when quality filtering was done to achieve a quality threshold (q) of 30 and 22 are shown for Z. haageana and H. helianthoides datasets. N50 refers to the contig length where 50% of the assembly is represented by contigs of this size or longer.