Fig. 1.

ABT-737 has a potent cytotoxic effect on bone marrow-derived AML pLSCs. Bone marrow-derived primary AML cells were cultured on a stromal-feeder layer for 24 h and then treated with 30 nM and 300 nM of ABT-737 for 24 h. AML cell viability was evaluated using7-AAD staining and flow cytometry. (A) The cytotoxic effect of ABT-737 on the overall blast population. The graph shows the percentage of live cells for each sample. (B) ABT-737 is equally efficient in eradicating AML pLSCs. The graph shows the percentage of the CD34⁺/CD38⁻ cells within the surviving AML cell population in each sample. E Horizontal lines indicate mean values. Different shapes indicate data-points corresponding to particular patient samples (Patients cross-referencing with Table 1: Patient 1 (○), Patient 2 (■), Patient 3 (•), Patient 4 (□)).