FIG. 8.

Adenoviral genome replication in A549 and human T-cell lines. All cells were infected with 30 PFU/cell of rec700 (A, B, and C) or dl309 (D) as described in Materials and Methods. The genome/PFU ratio for the CsCl-banded rec700 virus used in these experiments was 350. Thus, in each sample there were 5 × 10⁹ input genomes as measured by real-time PCR for hexon. Real-time PCR was used to quantitate genome copy numbers at the noted times p.i. (A, B, and C). (D) In separate experiments, Southern blotting was used to confirm replication of viral DNA in infected lymphocytes. Lane m, mock-infected cells. The approximate migration of molecular size standards in kilobase pairs is indicated to the right of each blot.