TABLE 2.
Characteristics of NeleNPV ORFse
| ORF no. | Name | Position | Size (aa) | AcMNPV ORF no. (% aa id)a | HaSNPV ORF no. (% aa id) | CuniNPV ORF no. (% aa id) | PxGV ORF no. (% aa id) | Comment(s)b |
|---|---|---|---|---|---|---|---|---|
| 1 | Polyhedrin | 1>744 | 247 | 8 (46.7) | 1 (45.9) | X | 4 (36.7) | NeabNPVpolyhedrin, e = e − 143, aa id 98% |
| 2 | 961>1287 | 108 | Internal repeats | |||||
| 3 | 1680>1922 | 80 | ||||||
| 4 | 1843>2841 | 332 | ||||||
| 5 | 3049<3207 | 52 | ||||||
| 6 | 3671<4450 | 259 | Trypsin-like serine protease, AAD21829 C. felis CfSP-2, e = 8e − 46, aa id 38.7% | |||||
| 7 | 4663<5058 | 131 | Internal repeat | |||||
| Direct repeat 1 | 5152-5194 | 2 43-bp direct repeats | ||||||
| 5223-5265 | ||||||||
| 8 | 5279<6859 | 526 | Internal repeat | |||||
| 9 | 7567>9951 | 794 | ||||||
| 10 | 10181>10345 | 54 | ||||||
| 11 | iap-3 | 10334<11116 | 260 | X | 103 (23.8) | X | 98 (17.6) | Sf iap AF186378, e = 3e − 33, aa id 28.4% |
| 12 | 11194>11436 | 80 | ||||||
| 13 | 11733<12476 | 247 | Transmembrane domain, coiled coil | |||||
| 14 | dbp | 12565<13275 | 236 | 25 (18.6) | 25 (17.3) | X | 61 (14.3) | HaSNPV ORF25 dbp, e = 0.94, aa id 17.3%c |
| 15 | lef-11 | 13256<13561 | 101 | 37 (16.7) | 32 (23.5) | X | 46 (12.4) | LsNPV lef 11, e = 0.014, aa id 23.5% |
| 16 | 13575<14345 | 256 | 92 (21.4) | 80 (23.1) | 14 (15.6) | 76 (20.3) | MacoNPV B ORF 92, e = 2e −22, aa id 22.5% | |
| 17 | 14347>14859 | 170 | 93 (14.8) | 81 (18.4) | X | 75 (15.9) | LsNPV ORF93, e = 0.037, aa id 14.0% | |
| 18 | 14868>15518 | 216 | Signal peptide | |||||
| 19 | 15584<15775 | 63 | Transmembrane domain | |||||
| 20 | dna pol | 15806<18577 | 923 | 65 (25.8) | 67 (27.3) | 91 (15) | 93 (23.7) | MacoNPV B ORF114 DNA polymerase, e = 2e − 61, aa id 28.1% |
| 21 | 18576>20762 | 728 | 2 coiled coils | |||||
| 22 | 20927>22069 | 380 | ||||||
| 23 | odv-e56 | 22321>23331 | 336 | 148 (32.6) | 15 (38) | 102 (20.8) | 16 (37.7) | PhopGV ORF 16 odv-e56, e = 1e − 54, aa id 37.7% |
| 24 | 23386>23964 | 192 | ||||||
| 25 | 24120<24905 | 261 | ||||||
| Direct repeat 2 | 24924-25029 | 2 53-bp direct tandem repeats | ||||||
| 26 | 25183<25863 | 226 | ||||||
| 27 | 26082>26654 | 190 | ||||||
| 28 | p6.9 | 26772<27080 | 102 | 100 (30.4) | 88 (23.3) | 23 (34.8) | 67 (40.4) | AdorGV ORF 72 p6.9, aa id 64.3%d |
| 29 | p40 | 27110<28210 | 366 | 101 (9.4) | 89 (10.4) | X | 66 (11.4) | XcGV ORF 93 p40, aa id 13.9% |
| 30 | 28231<28581 | 116 | ||||||
| 31 | p48 | 28578<29750 | 390 | 103 (11.1) | 91 (14.8) | X | 63 (12.7) | MacoNPV A ORF 83 p48, e = 0.026, aa id 15.1% |
| 32 | 29769<30485 | 238 | 106/107 (8.1) | 101 (18.4) | X | 40 (17.9) | LdMNPV ORF140, e = 6e − 10, aa id 16.7% | |
| 33 | alk-exo | 30588>31796 | 402 | 133 (24.3) | 114 (19.6) | 54 (16.8) | 106 (23.0) | PxGV ORF106 alk exo, e = 3e − 25, aa id 23.0% |
| 34 | 31793>32266 | 157 | Transmembrane domain, coiled coil | |||||
| 35 | 32364<32657 | 97 | Internal repeats | |||||
| 36 | 32787<33746 | 319 | ||||||
| 37 | lef-9 | 33771<35282 | 503 | 62 (31.5) | 55 (33.5) | 59 (17.1) | 99 (32.3) | PhopGV ORF 109 lef-9, e = 2e − 75, aa id 34.1% |
| 38 | 35308<35733 | 141 | 68 (15.5) | 64 (21.6) | 58 (18.1) | 96 (17.8) | SpltNPV ORF66, e = 2e − 04, aa id 21.8%, signal peptide, transmembrane domain | |
| Direct repeat 3 | 35829-35886 | 2 29-bp direct tandem repeats | ||||||
| 39 | 35984>37045 | 353 | ||||||
| 40 | 37042<37419 | 125 | ||||||
| 41 | 37416<37646 | 76 | 76 (18.2) | 70 (19.5) | X | 91 (22.1) | SeNPV ORF95, e = 1.4, aa id 24.7%d | |
| 42 | vlf-1 | 37649<38713 | 354 | 77 (22.8) | 71 (28.5) | 18 (17.5) | 89 (28.8) | SpltNPV ORF74, vlf-1, e = 1e − 41, aa id 29.6% |
| 43 | 38682<38966 | 94 | 78 (14.7) | 72 (20.0) | X | 88 (13.5) | AdorGV ORF 90, aa id 20.9%d | |
| 44 | gp41 | 38987<39799 | 270 | 80 (26.9) | 73 (30.6) | 33 (7.4) | 87 (20.3) | HaSNPV ORF 73 gp41, e = 2e − 19 |
| 45 | 39821>40348 | 175 | 81 (34.1) | 74 (35.2) | 106 (17.0) | 86 (34.7) | LdMNPV ORF 89, e = 5e − 23, aa id 34.1% | |
| 46 | p47 | 40601<41770 | 389 | 40 (21.3) | 35 (19.2) | 73 (8.5) | 51 (21.7) | CpGV ORF68 p47, e = 9e − 25, aa id 22.8% |
| 47 | p74 | 41772<43673 | 633 | 138 (38.3) | 20 (36.4) | 74 (34.4) | 49 (32.8) | RoMNPV ORF138 p74, e = e − 124, aa id 39.0% |
| 48 | 43651<44187 | 178 | ||||||
| 49 | 44247>44903 | 218 | Dm NP_609448, e = 1e − 13, aa id 17.4%, 4 C2H2 zinc fingers | |||||
| 50 | 45012>46235 | 407 | NeseNPV AF121349, e = 2e − 29, aa id 45.7%, internal repeat | |||||
| 51 | 46237>46815 | 192 | ||||||
| 52 | pif-2 | 46834>47988 | 384 | 22 (44.6) | 132 (43.8) | 38 (43.9) | 37 (47.2) | NeseNPV AF121349, e = e − 152, aa id 74.3% |
| 53 | 48058>48372 | 104 | ||||||
| 54 | lef-2 | 48374>48961 | 195 | 6 (13.8) | 117 (16.3) | 25 (14.8) | 32 (11.2) | NeseNPV AF121349 lef-2, e = 7e − 27, aa id 35.7% |
| 55 | lef-5 | 48989<49696 | 235 | 99 (25.4) | 87 (25.8) | 88 (9.3) | 69 (28.4) | NeseNPV AF121349 lef-5, e = 2e − 66, aa id 58% |
| 56 | 38k | 49687>50604 | 285 | 98 (30.1) | 86 (27.3) | 87 (25.5) | 70 (30.4) | OpMNPV ORF99 38k, e = 8e − 27, aa id 26.2% |
| 57 | 50590<51096 | 168 | 96 (25.4) | 85 (27.2) | 90 (26) | 71 (23.5) | CuniNPV ORF 90, e = 3e − 16, aa id 26.0% | |
| 58 | helicase | 51083>54487 | 1134 | 95 (16.8) | 84 (20.4) | 89 (11.9) | 72 (17.8) | PxGV ORF72 helicase, e = 7e − 45 |
| 59 | lef-4 | 54484<55890 | 468 | 90 (20.9) | 79 (22.1) | 96 (16) | 78 (25.9) | PxGV ORF78 lef-4, e = 2e − 28 |
| 60 | p49 | 55904>57232 | 442 | 142 (20.5) | 9 (22.1) | 30 (12.4) | 14 (16.9) | HaSNPV ORF 9 p49, e = 1e − 22 |
| 61 | 57244>57480 | 78 | transmembrane domain | |||||
| 62 | odv-e18 | 57503>57760 | 85 | 143 (14.3) | 10 (14.6) | X | 13 (24.4) | XcGV ORF 12 odv-e18, aa id 27.4%d |
| 63 | odv-ec27 | 57775>58563 | 262 | 144 (21.3) | 11 (18.3) | 32 (9.1) | 80 (16.0) | BmNPV ORF120 odv-e27, e = 1e − 05, aa id 21.7% |
| 64 | 58590>58922 | 110 | 145 (24.4) | 12 (23.7) | X | 12 (23.2) | CpGV ORF9, e = 2e − 07, aa id 30.4% | |
| 65 | lef-1 | 58925<59560 | 211 | 14 (19.8) | 124 (29.2) | 45 (28.8) | 55 (29.2) | PhopGV ORF 66 lef-1, e = 9e − 16, aa id 30.7% |
| 66 | 59622>60203 | 193 | 115 (26.3) | 98 (30.4) | 46 (25.3) | 29 (28.0) | SpltNPV ORF107, e = 1e − 12, aa id 25.8% | |
| 67 | 60357>61421 | 354 | 109 (20.0) | 54 (18.0) | 69 (16.1) | 43 (17.7) | MacoNPV A ORF 80, e = 1e − 13, aa id 22.5% | |
| 68 | 61421>61630 | 69 | Signal peptide | |||||
| 69 | 61617>62027 | 136 | RCC1/BLIP-II | |||||
| 70 | 61993>62394 | 133 | RCC1/BLIP-II | |||||
| 71 | 62479>62778 | 99 | RCC1/BLIP-II | |||||
| 72 | 63034>63885 | 283 | ||||||
| 73 | 63835<64128 | 97 | Transmembrane domain | |||||
| 74 | 64127>64924 | 265 | ||||||
| 75 | 65298>65927 | 209 | ||||||
| 76 | pif | 65909<67501 | 530 | 119 (26.9) | 111 (28.5) | 29 (28.2) | 7 (28.1) | AcMNPV ORF119 pif, e = 2e − 59 |
| Direct repeat 4 | 67561-67606 | 3 46-bp direct repeats | ||||||
| 67652-67697 | ||||||||
| 67742-67787 | ||||||||
| 77 | 67897<68346 | 149 | 53 (13.6) | 43 (18.2) | X | 112 (11.6) | CpGV ORF 134, e = 2.4 aa id 15.7%c | |
| 78 | lef-8 | 68351>70882 | 843 | 50 (29.7) | 38 (30.6) | 26 (19.7) | 109 (32.1) | PhopGV ORF 121 lef-8, e = e − 113, aa id 33.9% |
| Direct repeat 5 | 71364-71562 | 3 67-bp direct tandem repeats | ||||||
| Direct repeat 6 | 72017-72105 | 2 45-bp direct tandem repeats | ||||||
| 79 | 72085<72279 | 64 | ||||||
| 80 | 72547>72783 | 78 | 2 transmembrane domains | |||||
| 81 | 72879>73325 | 148 | Casphalia extranea densovirus NP_694840, e = 1e − 11, aa id 30.2% | |||||
| 82 | vp91 capsid | 73334>75745 | 803 | 83 (22.8) | 76 (22.9) | 35 (21.6) | 84 (23.4) | HaSNPV ORF77 vp91 capsid, e = 3e − 56 |
| 83 | vp1054 | 75747<76688 | 313 | 54 (20.4) | 47 (17.5) | 8 (15.0) | 115 (15.4) | LdMNPV ORF57 vp1054, e = 2e − 09, aa id 19.1% |
| 84 | 76813>77022 | 69 | ||||||
| 85 | 77030>77947 | 305 | ||||||
| Direct repeat 7 | 77914-77943 | 2 30-bp repeats, one within nl85 | ||||||
| 78383-78412 | ||||||||
| Direct repeat 8 | 78002-78161 | 2 160-bp direct tandem repeats overlapping nl86 | ||||||
| 78162-78321 | ||||||||
| 86 | 78101<78361 | 86 | Signal peptide, two transmembrane domains | |||||
| 87 | 78468>79898 | 476 | Internal repeat | |||||
| Direct repeat 9 | 79967-80009 | 2 43-bp direct repeats | ||||||
| 80056-80098 | ||||||||
| 88 | vp39 capsid | 80133<81080 | 315 | 89 (18.7) | 78 (17.7) | 24 (11.0) | 79 (14.2) | SpltNPV ORF81 p39, e = 7e − 20, aa id 20.5% |
| 89 | 81089>81640 | 183 | Phosphotransferase | |||||
| Avg (23.2) | Avg (24.7) | Avg (19.7) | Avg (23.6) |
a
% Amino acid identity (aa id) calculated for complete ORFs using ClustalW.
b
Top NCBI blastp 2.2.5 or SMART WU-BLAST 2.0 match with e-value and % aa id. If top % aa id is listed in columns 5 to 8, %, it is not repeated. In some instances top blastp match and ORF with highest % aa id are not the same; if no baculovirus homologue was clearly identified, domains and features as identified with SMART or NCBI blast conserved domain database are given.
c
Accepted based on NeleNPV results.
d
Accepted based on aa id of >20%.
e
ORFS in bold are considered clearly identified using criteria given in Materials and Methods. < and > indicate direction of transcription. e values are given as follows: e = 8e − 46, e = 8 × 10−46.