FIG. 3.

Flow cytometric analysis of CAV9 binding to SW480 cells expressing α_vβ₃ or α_vβ₆ and enhanced growth of CAV9 in cells expressing α_vβ₆. (A) Control cell samples were processed in the absence of virus (filled histogram). Virus binding (open histogram) was detected on SW480-α_vβ₆. Virus binding was not detected with SW480-mock or SW480-α_vβ₃ cells. (B) Levels (in PFU) of CAV9 and the RGD-less derivative D4 obtained when grown on monolayers of SW480-α_vβ₃ (b3), SW480-α_vβ₆ (b6), and SW480-mock (mock) cells for 72 h. The input virus inoculum is also shown (labeled “original”). The results are an average of duplicate measurements.