FIG. 5.

GLS1 silencing increased NPC apoptosis. Human NPCs were transfected by control siRNA or GLS1 siRNA, then maintained in growth media for 3 days. NPC apoptosis was determined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. (A) Representative pictures of TUNEL⁺ (green) cells in control siRNA- and siGLS1-transfected NPC culture were shown. Nestin (red) was used to label NPCs. Nuclei (blue) were labeled with DAPI. (B) The percentage of TUNEL⁺ cells was determined by counting the number of TUNEL⁺ cells over the number of DAPI⁺ cells in each microscope field. Images were acquired from a Nikon Eclipse TE2000E fluorescent microscope. Magnifications: 20× objective lens. Scale bar=50 μm. Data are shown as mean±SEM of 10 fields in each experimental group for the three donors. *P<0.05 compared with the control siRNA group, n=3. Color images available online at www.liebertpub.com/scd