FIG. 8.

Different patterns of mitochondrial accumulation in iCSCs. U87 iCSCs showing two different configurations of mitochondrial accumulation, a polarized tail-like structure (A, B), and a shell-like arrangement (C–E). Formation of methuosis-like process was observed as a preamble to cytoplasm shedding (C–E), with the nuclei entraining some cytoplasmic material in their intermembrane space (F). NB69 iCSCs showing perinuclear accumulation of mitochondria, shedding of cytoplasm, and nuclei encapsulated for a hibernation period (G–L). De novo colonies in NGP neuroblastoma (M, N) and U87 iCS glioblastoma cells (O). Magnification: (F) 3000×. Arrows indicate mitochondrial accumulation.