FIG. 2.
AT-2-inactivated or infectious R5 HIV-1 does not inhibit B-cell responses in human lymphoid tissue ex vivo. Tissue blocks challenged with TT and PWM were inoculated with AT-2-inactivated or infectious R5SF162 virus. The antibody response was evaluated as anti-TT or total IgG (mean ± standard error) released by 27 to 36 identically treated tissue blocks from each donor and expressed as percent relative to similarly challenged matched sham-treated tissue. Concentrations of inactivated virus are indicated: 1× = 15-ng/ml p24gag, the peak concentration in the medium of productively infected tissue cultures. (a) Production of anti-TT IgG in tissues challenged with TT and inoculated with infectious R5SF162 or AT-2-inactivated R5SF162 at 1× or 10× (n = 6, 6, and 7 donor tissues, respectively). (b) Production of IgG in tissues challenged with PWM and inoculated with infectious R5SF162 or AT-2-inactivated R5SF162 at 1× or 10× (n = 9, 9, and 7 donor tissues, respectively).