FIG. 1.

Immune and functional phenotypes of ADSCs and fibroblasts. (A) Analysis of immune phenotypes by flow cytometry. ADSCs (gray lines) and fibroblasts (black lines) were labeled with antibodies against the indicated antigens, and analyzed by flow cytometry. Representative histograms are displayed. Isotype controls are shown as empty lines/areas, and the solid histograms indicate reactivity with the antibody. (B) Differentiation of ADSCs and fibroblasts. Both cell types at passage P5 were incubated for 21 days in the presence of specific agents inducing differentiation into adipocytes. Differentiation into the adipocyte lineage was demonstrated by staining with Oil Red O. Untreated control cultures without adipogenic differentiation stimuli are shown on the upper line of photographs. The scale bar indicates 200 μm. ADSCs, adipose-derived stromal cells.