FIG. 1.
Expression of Rad51 throughout the cell cycle in mouse embryonic stem cells (mESCs). (A) Rad51 protein levels in mESCs and mouse embryonic fibroblasts (MEFs). mESCs were spontaneously differentiated by removing leukemia inhibitory factor (LIF) and adding retinoic acid (0.2 μM). (B) Quantification of Rad51 expression in mESCs and MEFs. (C) mESCs were synchronized with a double thymidine and then released from G1/S phase. The cells were collected at 2.5-h intervals, as indicated. Cyclin B1/A and phospho-histone H3Ser10 were used as markers for cell cycle progression. α-Tubulin was used as a loading control. (D) The level of each protein was quantified. Relative ratio of each protein band over the band of α–tubulin was described in each time point. The numerical value of each sample at indicated time point was normalized by the value of asynchronous cells (As). (E) Cell cycle profile was assessed by fluorescence-activated cell sorting (FACS) analysis.