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Morphology, viability, and proliferation of three-dimensional (3D)-murine embryonic stem cells (mESCs). (A) Representative mESC-beads on (i) day 3 and (ii) day 20. Magnification: 4×; Scale: 500 μm. (iii) day 20 viability in composite images of Calcien AM (iii’) and nonviable cells (Ethidium homodimer-1;) (iii’’); negative controls in insets. Magnification: 20×. Scale: 200 μm. (B) Cell proliferation over 21 days [mean number of cells±standard deviation (SD); n=3, *P≤0.05].
