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Gene and protein assessment of 3D alginate-encapsulated mESCs. (A) Time course of gene expression by reverse transcriptase-polymerase chain reaction. Negative controls consisted of sample without cDNA. [M]=maintenance stage. (B) Immunohistochemistry of erythroid markers CD71 and TER119 at day 15 of culture. Negative controls in insets. Magnification: 20×. Scale: 200 μm.
